welcome again in Meet the chickens section. Today a specific labelling of chromosomes (actually sister chromatids) will be presented.
As you probably remember from previous post (Meet the chickens – chook chromosomes at hand), there is a way to examine karyotype (set of chromosomes) of chicken DT40 cell line (or any other cell line:). In this post we will combine this technique with a labelling procedure. This protocol will give us oportunity to see differential staining of sister chromatids.
Ok lets go.
In this protocol a nucleotide derivative, called BrdU (5-bromo deoxyuridine) is used to labell DNA for two cell cycles (see the picture below).
