welcome again in Meet the chickens section. Today a specific labelling of chromosomes (actually sister chromatids) will be presented.

As you probably remember from previous post (Meet the chickens – chook chromosomes at hand), there is a way to examine karyotype (set of chromosomes) of chicken DT40 cell line (or any other cell line:). In this post we will combine this technique with a labelling procedure. This protocol will give us oportunity to see differential staining of sister chromatids.

Ok lets go.

In this protocol a nucleotide derivative, called BrdU (5-bromo deoxyuridine) is used to labell DNA for two cell cycles (see the picture below).


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