This is a nitrate ester made by esterification. In this process, glycerols reacts with Nitric Acid under a catalyst of Sulphuric Acid at 300oC to yield an ester complex of glyceryl trinitrate otherwise known as nitroglycerine, and three water molecules. In this process, there has been formation of an ester bond (COO). This production is very crucial for medical purposes. Nitroglycerine is used to relieve angina, a condition when the heart isn’t receiving much oxygen. Also, Nitroglycerine was used in a study with yohimbine/l-arginine combination to see the treatment of erectile dysfunction in males. The metrics used in this study were the male’s systolic and diastolic blood pressures. This study created a correlation between the effects of erectile dysfunction with the inadvertent cause of coronary artery disease. Intravenous Nitroglycerine was used to help in the study with the chemical compositions with arginine.